Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir, . and Lai, Sin Yuan and Azren Aida, Asmawi and Chew, Kit Wayne and Ngan, Cheng Loong (2024) Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp. Bioresource Technology, 393 (130094). pp. 1-8. ISSN 0960-8524. (Published)

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Abstract

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

Item Type:	Article
Additional Information:	Indexed by Scopus
Uncontrolled Keywords:	Aqueous two-phase system; Biorefinery; Chlorella vulgaris; Rotor-stator homogenization; Separation
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology
Depositing User:	Mrs Norsaini Abdul Samat
Date Deposited:	02 Dec 2024 07:07
Last Modified:	02 Dec 2024 07:07
URI:	http://umpir.ump.edu.my/id/eprint/42995
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