Norhalmy, Mohd Nor (2006) Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
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Abstract
ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB.
| Item Type: | Undergraduates Project Papers |
|---|---|
| Additional Information: | For FULL TEXT, email ump.pap@gmail.com Project paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006 |
| Uncontrolled Keywords: | Fermentation Sugarcane |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Rosfadilla Mohamad Zainun |
| Date Deposited: | 19 Apr 2010 06:42 |
| Last Modified: | 03 Jun 2021 05:17 |
| URI: | http://umpir.ump.edu.my/id/eprint/559 |
| Download Statistic: | View Download Statistics |
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