Syed Mohd Saufi, Tuan Chik and Fee, Conan J. (2013) Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation. Journal of Membrane Science, 444. pp. 157-163. ISSN 0376-7388. (Published)
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The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Membrane chromatography; Hydrophobic interaction; Whey protein; Mixed matrix membrane |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Mrs. Neng Sury Sulaiman |
| Date Deposited: | 27 Aug 2015 03:10 |
| Last Modified: | 11 Apr 2018 01:57 |
| URI: | http://umpir.ump.edu.my/id/eprint/6215 |
| Download Statistic: | View Download Statistics |
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