Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source

Abeed Fatima, Mohidin Batcha and D. M. R., Prasad and Khan, Maksudur R. and Hamidah, Abdullah (2014) Biosynthesis of Poly (3-hydroxybutyrate)(PHB) by Cupriavidus Necator H16 from Jatropha Oil as carbon source. Bioprocess and Biosystems Engineering, 37 (5). pp. 943-951. ISSN 1615-7591 (print); 1615-7605 (online). (Published)

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Abstract

Poly(3-hydroxybutyrate) (PHB) is a biodegradable polymer that can be synthesized through bacterial fermentation. In this study, Cupriavidus necator H16 is used to synthesize PHB by using Jatropha oil as its sole carbon source. Different variables mainly jatropha oil and urea concentrations, and agitation rate were investigated to determine the optimum condition for microbial fermentation in batch culture. Based on the results, the highest cell dry weight and PHB concentrations of 20.1 and 15.5 g/L, respectively, were obtained when 20 g/L of jatropha oil was used. Ethanol was used as external stress factor and the addition of 1.5 % ethanol at 38 h had a positive effect with a high PHB yield of 0.987 g PHB/g jatropha oil. The kinetic studies for cell growth rate and PHB production were conducted and the data were fitted with Logistic and Leudeking–Piret models. The rate constants were evaluated and the theoretical values were in accordance with the experimental data obtained.

Item Type:	Article
Uncontrolled Keywords:	Cupriavidus necator H16; External stress factor; Jatropha oil; Poly(3-hydroxybutyrate)(PHB); Kinetic model Li
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering
Depositing User:	Mrs. Neng Sury Sulaiman
Date Deposited:	07 Jan 2015 02:13
Last Modified:	15 Dec 2017 08:06
URI:	http://umpir.ump.edu.my/id/eprint/8060
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