Nur Amira, Maarof (2014) Production of poly (3-hydroxybutyrate) from pineapple waste. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
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Abstract
P(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics. Thus, the aim of this research is to employ the sugar present in pineapple waste as the carbon source for the bacteria producing biopolymer. The objectives are as follow; to determine the sugar content in the pineapple waste juice, to study the effect percent volume of juice from pineapple waste on production of P(3HB), to study the effect of pH on P(3HB) production. The method involve in this study are cultivation of bacteria, preparation of pineapple waste, preparation of inoculum, P(3HB) biosynthesis in shake flask, cell dry weight analysis and analysis of P(3HB) using HPLC. The results showed that glucose content in the mixture of skins and pulps is the highest with total of 28.80 g/L compared to fructose and sucrose. Fructose content come second with 21.25 g/L while sucrose has the lowest content in the PWJ with 20.28 g/L. The pineapple waste juice (PWJ) is then used as medium for the production of P(3HB). The fermentation medium with 50% and 100% volume of PWJ has the highest concentration of P(3HB) of 2.01 g/L (30th hour) and 1.49 g/L (44th hour) respectively. As the total dry mass production, the medium with 75% of PWJ has the highest concentration of P(3HB) with 14.42 g/L. The total concentration of P(3HB) for 50% and 100% PWJ are 10.52 g/L and 4.42 g/L respectively. The total average concentration of P(3HB) produced in the medium at pH 7 is 8.97 g/L. The highest concentration was found to be at the 44th hours with 2.15 g/L. As conclusion, the medium that consists of 75% PWJ and 25% ultra-pure water with the mixture of nutrient rich element at pH 7 is the most suitable medium to produce P(3HB).
| Item Type: | Undergraduates Project Papers |
|---|---|
| Additional Information: | Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2014, SV: DR. MIOR AHMAD KHUSHAIRI MOHD ZAHARI, NO CD: 8659 |
| Uncontrolled Keywords: | Biopolymers; Fermentations |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Mr. Nik Ahmad Nasyrun Nik Abd Malik |
| Date Deposited: | 16 Oct 2015 00:05 |
| Last Modified: | 15 Nov 2023 02:46 |
| URI: | http://umpir.ump.edu.my/id/eprint/9158 |
| Download Statistic: | View Download Statistics |
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