Tan, Darlene Tzet Lene (2017) Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry. Faculty of Engineering Technology, Universiti Malaysia Pahang.
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Abstract
The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at
| Item Type: | Undergraduates Project Papers |
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| Additional Information: | Project Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2018, SV: DR. SITI UMAIRAH BINTI MOKTHAR, NO. CD: 11344 |
| Uncontrolled Keywords: | Propolis; TLC; HPLC; UV VIS spectrophotometry |
| Subjects: | T Technology > T Technology (General) |
| Faculty/Division: | Faculty of Engineering Technology |
| Depositing User: | Mrs. Sufarini Mohd Sudin |
| Date Deposited: | 29 May 2019 04:52 |
| Last Modified: | 20 Nov 2023 07:56 |
| URI: | http://umpir.ump.edu.my/id/eprint/24521 |
| Download Statistic: | View Download Statistics |
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